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Isolation and characterization of microsatellite loci from Arthropodium cirratum (Asparagaceae).

ABSTRACT: Microsatellite markers were developed for Arthropodium cirratum (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross-amplification in two other Arthropodium species. Sixteen microsatellite markers were developed from a genomic library and tested in three populations of A. cirratum. The loci exhibited one to five alleles per locus, with private alleles present in each of the populations. Cross-amplification tests in the two other New Zealand Arthropodium species revealed that many of the loci amplify and demonstrate polymorphism in A. bifurcatum. These markers will be useful for determining genetic structure in A. cirratum and for determining the origins of translocated populations of this species.

SUBMITTER: Bulgarella M

PROVIDER: S-EPMC5584818 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature

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Isolation and characterization of microsatellite loci from <i>Arthropodium cirratum</i> (Asparagaceae).

Bulgarella Mariana M Biggs Patrick J PJ de Lange Peter J PJ Shepherd Lara D LD

Applications in plant sciences 20170825 8

<h4>Premise of the study</h4>Microsatellite markers were developed for <i>Arthropodium cirratum</i> (Asparagaceae) to study population genetic structure and translocation of this species. These markers were tested for cross-amplification in two other <i>Arthropodium</i> species.<h4>Methods and results</h4>Sixteen microsatellite markers were developed from a genomic library and tested in three populations of <i>A. cirratum</i>. The loci exhibited one to five alleles per locus, with private allele ...[more]

PMID: 28924514

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