The chromatin-remodeling factor CHD4 is required for maintenance of childhood acute myeloid leukemia.

ABSTRACT: Epigenetic alterations contribute to leukemogenesis in childhood acute myeloid leukemia and therefore are of interest for potential therapeutic strategies. Herein, we performed large-scale ribonucleic acid interference screens using small hairpin ribonucleic acids in acute myeloid leukemia cells and non-transformed bone marrow cells to identify leukemia-specific dependencies. One of the target genes displaying the strongest effects on acute myeloid leukemia cell growth and less pronounced effects on nontransformed bone marrow cells, was the chromatin remodeling factor CHD4 Using ribonucleic acid interference and CRISPR-Cas9 approaches, we showed that CHD4 was essential for cell growth of leukemic cells in vitro and in vivo Loss of function of CHD4 in acut

SUBMITTER: Heshmati Y 

PROVIDER: S-EPMC6029541 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature