Selection of reference genes for quantitative real-time PCR analysis in halophytic plant Rhizophora apiculata.

ABSTRACT: Rhizophora apiculata is a halophytic, small mangrove tree distributed along the coastal regions of the tropical and subtropical areas of the world. They are natural genetic reservoirs of salt adaptation genes and offer a unique system to explore adaptive mechanisms under salinity stress. However, there are no reliable studies available on selection and validation of reference genes for quantitative real-time polymerase chain reaction (qRT-PCR) in R. apiculata physiological tissues and in salt stress conditions. The selection of appropriate candidate reference gene for normalization of qRT-PCR data is a crucial step towards relative analysis of gene expression. In the current study, seven genes such as elongation factor 1α (EF1α), Ubiquitin (UBQ), β-tubulin (β-

SUBMITTER: Saddhe AA 

PROVIDER: S-EPMC6046198 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature