MSI2 knockdown represses extrahepatic cholangiocarcinoma growth and invasion by inhibiting epithelial-mesenchymal transition.

ABSTRACT:

Purpose

To investigate the expression and functional role of Musashi2 (MSI2), an RNA-binding protein, in extrahepatic cholangiocarcinoma (eCCA).

Patients and methods

We measured MSI2 expression in human specimens and cell lines using Western blot and quantitative real-time polymerase chain reaction, and we analyzed its association with clinicopathologic features in eCCA patients. Univariate and multivariate analyses were performed to identify risk factors correlated with overall survival and disease-free survival. Functional experiments were used to study the mechanisms of MSI2 in regulating eCCA cell growth, migration, and invasion.

Results

MSI2 expression was upregulated significantly in both human specimens and cell lines, and high MSI2 expression was associated wi

SUBMITTER: Hu F 

PROVIDER: S-EPMC6049051 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature