Multiplex immunohistochemistry accurately defines the immune context of metastatic melanoma.

ABSTRACT: A prospective study explored the heterogeneous nature of metastatic melanoma using Multiplex immunohistochemistry (IHC) and flow cytometry (FACS). Multiplex IHC data quantitated immune subset number present intra-tumoral (IT) vs the tumor stroma, plus distance of immune subsets from the tumor margin (TM). In addition, mIHC showed a close association between the presence of IT CD8⁺ T cells and PDL1 expression in melanoma, which was more prevalent on macrophages than on melanoma cells. In contrast, FACS provided more detailed information regarding the T cell subset differentiation, their activation status and expression of immune checkpoint molecules. Interestingly, mIHC detected significantly higher Treg numbers than FACS and showed preferential CD4⁺ T cell distributio

SUBMITTER: Halse H 

PROVIDER: S-EPMC6057961 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature