Mitochondrial genomes organization in alloplasmic lines of sunflower (Helianthus annuus L.) with various types of cytoplasmic male sterility.

ABSTRACT:

Background

Cytoplasmic male sterility (CMS) is a common phenotype in higher plants, that is often associated with rearrangements in mitochondrial DNA (mtDNA), and is widely used to produce hybrid seeds in a variety of valuable crop species. Investigation of the CMS phenomenon promotes understanding of fundamental issues of nuclear-cytoplasmic interactions in the ontogeny of higher plants. In the present study, we analyzed the structural changes in mitochondrial genomes of three alloplasmic lines of sunflower (Helianthus annuus L.). The investigation was focused on CMS line PET2, as there are very few reports about its mtDNA organization.

Methods

The NGS sequencing, de novo assembly, and annotation of sunflower mitochondrial genomes were performed. The comparati

SUBMITTER: Makarenko MS 

PROVIDER: S-EPMC6061164 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature