Ontology highlight
ABSTRACT:
SUBMITTER: Vian L
PROVIDER: S-EPMC6065110 | biostudies-literature | 2018 May
REPOSITORIES: biostudies-literature
Cell 20180426 5
Cohesin extrusion is thought to play a central role in establishing the architecture of mammalian genomes. However, extrusion has not been visualized in vivo, and thus, its functional impact and energetics are unknown. Using ultra-deep Hi-C, we show that loop domains form by a process that requires cohesin ATPases. Once formed, however, loops and compartments are maintained for hours without energy input. Strikingly, without ATP, we observe the emergence of hundreds of CTCF-independent loops tha ...[more]