Characterization of UDP-Activated Purinergic Receptor P2Y₆ Involved in Japanese Flounder Paralichthys olivaceus Innate Immunity.

ABSTRACT: Uridine 5'-diphosphate (UDP)-activated purinergic receptor P2Y₆ is a member of a G-protein-coupled purinergic receptor family that plays an important role in mammalian innate immunity. However, the role of the P2Y₆ receptor (P2Y₆R) in fish immunity has not been investigated. In this report, we characterized a P2Y₆R gene from Japanese flounder (Paralichthys olivaceus) and examined its role in fish innate immunity. Sequence analysis reveals that the Japanese flounder P2Y₆R protein is conserved and possesses four potential glycosylation sites. Quantitative real-time RT-PCR analysis shows that P2Y₆R is broadly distributed in all examined Japanese flounder tissues with dominant expression in the liver. In addition, P2Y₆R gene expression was up-regulated in head kidney macrophages (HKMs) upon li

SUBMITTER: Li S 

PROVIDER: S-EPMC6073673 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature