Variation in FPOP Measurements Is Primarily Caused by Poor Peptide Signal Intensity.

ABSTRACT: Fast photochemical oxidation of proteins (FPOP) may be used to characterize changes in protein structure by measuring differences in the apparent rate of peptide oxidation by hydroxyl radicals. The variability between replicates is high for some peptides and limits the statistical power of the technique, even using modern methods controlling variability in radical dose and quenching. Currently, the root cause of this variability has not been systematically explored, and it is unknown if the major source(s) of variability are structural heterogeneity in samples, remaining irreproducibility in FPOP oxidation, or errors in LC-MS quantification of oxidation. In this work, we demonstrate that coefficient of variation of FPOP measurements varies widely at low peptide signal intensity, but stabil

SUBMITTER: Abolhasani Khaje N 

PROVIDER: S-EPMC6087495 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature