Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations.

ABSTRACT:

Background & aims

γδ T cells comprise a substantial proportion of tissue-associated lymphocytes. However, our current understanding of human γδ T cells is primarily based on peripheral blood subsets, while the immunobiology of tissue-associated subsets remains largely unclear. Therefore, we aimed to elucidate the T cell receptor (TCR) diversity, immunophenotype and function of γδ T cells in the human liver.

Methods

We characterised the TCR repertoire, immunophenotype and function of human liver infiltrating γδ T cells, by TCR sequencing analysis, flow cytometry, in situ hybridisation and immunohistochemistry. We focussed on the predominant tissue-associated Vδ2^- γδ subset, which is implicated in liver immunopathology.

Results

Intrahepatic Vδ2^- γδ

SUBMITTER: Hunter S 

PROVIDER: S-EPMC6089840 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature