Identification of Key Bacteria Involved in the Induction of Incident Bacterial Vaginosis: A Prospective Study.

ABSTRACT:

Background

The sequence of events preceding incident bacterial vaginosis (iBV) is unclear.

Methods

African American women who have sex with women, who had no Amsel criteria and Nugent scores of 0-3, were followed for 90 days to detect iBV (defined as a Nugent score of 7-10 on at least 2-3 consecutive days), using self-collected vaginal swab specimens. For women with iBV (cases) and women maintaining normal vaginal flora (healthy women), 16S ribosomal RNA gene sequencing targeting V4 was performed. Longitudinal vaginal microbiome data were analyzed.

Results

Of 204 women screened, 42 enrolled; of these, 45% developed iBV. Sequencing was performed on 448 specimens from 14 cases and 8 healthy women. Among healthy women, Lactobacillus crispatus dominated the vaginal microb

SUBMITTER: Muzny CA 

PROVIDER: S-EPMC6093354 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature