Neutrophil and T-Cell Homeostasis in the Closed Eye.

ABSTRACT:

Purpose

This study sought to examine the changes and phenotype of the tear neutrophil and T-cell populations between early eyelid closure and after a full night of sleep.

Methods

Fourteen healthy participants were recruited and trained to wash the ocular surface with PBS for at-home self-collection of ocular surface and tear leukocytes following up to 1 hour of sleep and a full night of sleep (average 7 hours), on separate days. Cells were isolated, counted, and incubated with fluorescently labeled antibodies to identify neutrophils, monocytes, and T cells. For neutrophil analysis, samples were stimulated with lipopolysaccharide (LPS) or calcium ionophore (CaI) before antibody incubation. Flow cytometry was performed.

Results

Following up to 1 hour of sleep, numerous

SUBMITTER: Postnikoff CK 

PROVIDER: S-EPMC6110127 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature