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ABSTRACT: Premise of the study
Polymorphic microsatellite markers were developed to study population structure and mating patterns of the monocarpic herb Moricandia moricandioides (Brassicaceae).Methods and results
Illumina MiSeq sequencing was used to develop a panel of 15 polymorphic microsatellite markers that were tested across 77 individuals from three populations on the Iberian Peninsula. All markers were polymorphic in at least two studied populations, and the number of alleles ranged from one to 11 per locus. The levels of observed and expected heterozygosity ranged from 0.000 to 1.000 and from 0.153 to 0.865, respectively. Nine and 11 loci were successfully amplified in the congeneric species M. arvensis and M. foetida, respectively.Conclusions
The 15 microsatellite markers will be useful for population genetic studies of the genus Moricandia. These markers will serve as a useful tool for exploring population structure and mating patterns of M. moricandioides.
SUBMITTER: Cuenot Y
PROVIDER: S-EPMC6110244 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature

Cuenot Yves Y Gómez José M JM González-Megias Adela A Pannell John R JR Torices Rubén R
Applications in plant sciences 20180821 8
<h4>Premise of the study</h4>Polymorphic microsatellite markers were developed to study population structure and mating patterns of the monocarpic herb <i>Moricandia moricandioides</i> (Brassicaceae).<h4>Methods and results</h4>Illumina MiSeq sequencing was used to develop a panel of 15 polymorphic microsatellite markers that were tested across 77 individuals from three populations on the Iberian Peninsula. All markers were polymorphic in at least two studied populations, and the number of allel ...[more]