A coupled chlorinase-fluorinase system with a high efficiency of trans-halogenation and a shared substrate tolerance.
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ABSTRACT:
SUBMITTER: Sun H
PROVIDER: S-EPMC6113055 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
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A coupled chlorinase-fluorinase system with a high efficiency of trans-halogenation and a shared substrate tolerance.
Chemical communications (Cambridge, England) 20180801 68
Enzymatic trans-halogenation enables radiolabeling under mild and aqueous conditions, but rapid reactions are desired. We developed a coupled chlorinase-fluorinase system for rapid trans-halogenation. Notably, the chlorinase shares a substrate tolerance with the fluorinase, enabling these two enzymes to cooperatively produce 5'-fluorodeoxy-2-ethynyladenosine (5'-FDEA) in up to 91.6% yield in 1 h. ...[more]
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