Dataset Information

Dataset describing ethanol and 1,2-propanediol production by a stenothermal moderately thermophilic anaerobe, Clostridium strain AK1.

ABSTRACT: The dataset details the fermentation of D-glucose, L-rhamnose, and L-fucose and their end-product formation by the moderate thermophile Clostridium strain AK1 (DSM 18778) as related to the work described in "Propanediol from L-rhamnose using the moderately thermophilic Clostridium strain AK1" [1]. The influence of culture conditions on end product formation from D-glucose and L-rhamnose by AK1 was investigated in batch culture. Strain AK1 was cultivated at initial substrate concentrations varying from 0 to 60 mM and initial pH values varying from 4.5 to 8.5. Additionally different cultivation temperatures (30-65 °C), the influence of liquid-gas phase ratio as well as different phosphate concentrations on growth were investigated.

SUBMITTER: Ingvadottir EM

PROVIDER: S-EPMC6127976 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Dataset describing ethanol and 1,2-propanediol production by a stenothermal moderately thermophilic anaerobe, Clostridium strain AK1.

Ingvadottir Eva Maria EM Scully Sean Michael SM Orlygsson Johann J

Data in brief 20180830

The dataset details the fermentation of D-glucose, L-rhamnose, and L-fucose and their end-product formation by the moderate thermophile Clostridium strain AK1 (DSM 18778) as related to the work described in "Propanediol from L-rhamnose using the moderately thermophilic Clostridium strain AK1" [1]. The influence of culture conditions on end product formation from D-glucose and L-rhamnose by AK1 was investigated in batch culture. Strain AK1 was cultivated at initial substrate concent ...[more]

PMID: 30197924

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Project description:The use of electronic cigarettes (e-cigarettes) is increasing despite insufficient information concerning their long-term effects, including the effects of maternal e-cigarette use on pre- and postnatal development. Our previous study demonstrated that developmental exposure to 1,2-propanediol (a principal component of e-cigarette liquid) affected early development of zebrafish, causing reduced growth, deformities, and hyperactive swimming behavior in larvae. The current study extends assessment of the developmental toxicity of 1,2-propanediol by examining additional long-term behavioral effects. We demonstrate that embryonic/larval exposure of zebrafish to 1,2-propanediol (0.625% or 1.25%) not only affected behavioral parameters in the larvae, but also caused persisting behavioral effects in adults after early developmental exposure. Additional parameters, including neural and vascular development in larvae, stress response in adults, and concentration of neurotransmitters dopamine and serotonin in adult brain were examined, in order to explain the behavioral differences. These additional assessments did not find 1,2-propanediol exposure to significantly affect Tg(Neurog1:GFP) or the transcript abundance of neural genes (Neurog1, Ascl1a, Elavl3, and Lef1). Vascular development was not found to be affected by 1,2-propanediol exposure, as inferred from experiments with Tg(Flk1:eGFP) zebrafish; however, transcript abundance of vascular genes (Flk1, Vegf, Tie-2, and Angpt1) was decreased. No statistically significant changes were noted for plasma cortisol or brain neurotransmitters in adult fish. Lastly, analysis of gene transcripts involved with 1,2-propanediol metabolism (Adh5, Aldh2.1, and Ldha) showed an increase in Adh5 transcript. This is the first study to demonstrate that developmental exposure to 1,2-propanediol has long-term neurobehavioral consequences in adult zebrafish, showing that e-cigarettes contain substances potentially harmful to neurodevelopment.

Dataset Information

Dataset describing ethanol and 1,2-propanediol production by a stenothermal moderately thermophilic anaerobe, Clostridium strain AK1.

Publications

Dataset describing ethanol and 1,2-propanediol production by a stenothermal moderately thermophilic anaerobe, <i>Clostridium</i> strain AK1.

Similar Datasets

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