Dataset Information

The draft genome sequence of Clostridium sp. strain LJ4 with high furan and phenolic derivates' tolerances occurring from lignocellulosic hydrolysates.

ABSTRACT: The genome of a wild-type solventogenic Clostridium sp. strain LJ4 that could directly convert undetoxified lignocellulosic hydrolysate to butanol and tolerate high concentration of furan and phenolic derivates occurring in the lignocellulosic hydrolysate is described. 16S rDNA gene sequencing and analysis indicated that it is closely related to Clostridium acetobutylicum. The genome size of strain LJ4 is 3.90 Mp, which has a G?+?C content of 30.72% and encodes 2711 proteins. It also has one 0.19 Mp plasmid with 181 predicted encoding proteins. Alcohol dehydrogenases (ADs) and a nicotinamide adenine dinucleotide phosphate (NADPH)-dependent flavin mononucleotide (FMN) reductase were identified, which may play key roles in inhibitors' resistance in lignocellulosic hydrolysate.

SUBMITTER: Liu J

PROVIDER: S-EPMC6138001 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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The draft genome sequence of Clostridium sp. strain LJ4 with high furan and phenolic derivates' tolerances occurring from lignocellulosic hydrolysates.

Liu Jie J Jiang Yujia Y Chen Tianpeng T Dong Weiliang W Zhang Wenming W Ma Jiangfeng J Jiang Min M Xin Fengxue F

3 Biotech 20180914 10

The genome of a wild-type solventogenic Clostridium sp. strain LJ4 that could directly convert undetoxified lignocellulosic hydrolysate to butanol and tolerate high concentration of furan and phenolic derivates occurring in the lignocellulosic hydrolysate is described. 16S rDNA gene sequencing and analysis indicated that it is closely related to Clostridium acetobutylicum. The genome size of strain LJ4 is 3.90 Mp, which has a G + C content of 30.72% and encodes 2711 proteins. It al ...[more]

PMID: 30237953

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Project description:BackgroundLignocellulosic biomass is a promising resource of renewable biochemicals and biofuels. However, the presence of inhibitors existing in lignocellulosic hydrolysates (LCH) is a great challenge to acetone-butanol-ethanol (ABE) fermentation by Clostridium acetobutylicum. In particular, phenolic compounds (PCs) from LCH severely block ABE production even at low concentrations. Thus, it is urgent to gain insight into the intracellular metabolic disturbances caused by phenolic inhibitors and elucidate the underlying mechanisms to identify key industrial bottlenecks that undermine efficient ABE production.ResultsIn this study, a time-course of ABE fermentation by C. acetobutylicum in the presence of four typical PCs (syringaldehyde, vanillin, ferulic acid, and p-coumaric acid) was characterized, respectively. Addition of PCs caused different irreversible effects on ABE production. Specifically, syringaldehyde showed the greatest inhibition to butanol production, followed by vanillin, ferulic acid, and p-coumaric acid. Subsequently, a weighted gene co-expression network analysis (WGCNA) based on RNA-sequencing data was applied to identify metabolic perturbations caused by four LCH-derived PCs, and extract the gene modules associated with extracellular fermentation traits. The hub genes in each module were subjected to protein-protein interaction analysis and enrichment analysis. The results showed that functional modules were PC-dependent and shared some unique features. Specifically, p-coumaric acid caused the most extensive transcriptomic disturbances, particularly affecting the gene expressions of ribosome proteins and the assembly of flagella, DNA replication, repair, and recombination; the addition of syringaldehyde caused significant metabolic disturbances on the gene expressions of ribosome proteins, starch and sucrose metabolism; vanillin mainly disturbed purine metabolism, sporulation and signal transduction; and ferulic acid caused a metabolic disturbance on glycosyl transferase-related gene expressions.ConclusionThis study uncovers novel insights into the inhibitory mechanisms of PCs for the first time and provides guidance for future metabolic engineering efforts, which establishes a powerful foundation for the development of phenol-tolerant strains of C. acetobutylicum for economically sustainable ABE production with high productivity from lignocellulosic biomass.

Dataset Information

The draft genome sequence of Clostridium sp. strain LJ4 with high furan and phenolic derivates' tolerances occurring from lignocellulosic hydrolysates.

Publications

The draft genome sequence of <i>Clostridium</i> sp. strain LJ4 with high furan and phenolic derivates' tolerances occurring from lignocellulosic hydrolysates.

Similar Datasets

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