Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression.

ABSTRACT:

Background

When developing CRISPR/Cas9 systems for crops, it is crucial to invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes, especially if the transformation system is difficult or time-consuming. Cotton is an important crop for the production of fiber, oil, and biofuel. However, the cotton stable transformation is usually performed using Agrobacterium tumefaciens taking between 8 and 12 months to generate T₀ plants. Furthermore, cotton is a heterotetraploid and targeted mutagenesis is considered to be difficult as many genes are duplicated in this complex genome. The application of CRISPR/Cas9 in cotton is severely hampered by the long and technically challenging genetic transformation process, making it imperative to maximize

SUBMITTER: Long L 

PROVIDER: S-EPMC6169012 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature