Distinct domains of the AVRPM3^A2/F2 avirulence protein from wheat powdery mildew are involved in immune receptor recognition and putative effector function.

ABSTRACT: Recognition of the AVRPM3^A2/F2 avirulence protein from powdery mildew by the wheat PM3A/F immune receptor induces a hypersensitive response after co-expression in Nicotiana benthamiana. The molecular determinants of this interaction and how they shape natural AvrPm3^a2/f2 allelic diversity are unknown. We sequenced the AvrPm3^a2/f2 gene in a worldwide collection of 272 mildew isolates. Using the natural polymorphisms of AvrPm3^a2/f2 as well as sequence information from related gene family members, we tested 85 single-residue-altered AVRPM3^A2/F2 variants with PM3A, PM3F and PM3F^L456P/Y458H (modified for improved signaling) in Nicotiana benthamiana for effects on recognition. An intact AvrPm3^a2/f2 gene was f

SUBMITTER: McNally KE 

PROVIDER: S-EPMC6175116 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature