Causality Analysis and Cell Network Modeling of Spatial Calcium Signaling Patterns in Liver Lobules.

ABSTRACT: Dynamics as well as localization of Ca²⁺ transients plays a vital role in liver function under homeostatic conditions, repair, and disease. In response to circulating hormonal stimuli, hepatocytes exhibit intracellular Ca²⁺ responses that propagate through liver lobules in a wave-like fashion. Although intracellular processes that control cell autonomous Ca²⁺ spiking behavior have been studied extensively, the intra- and inter-cellular signaling factors that regulate lobular scale spatial patterns and wave-like propagation of Ca²⁺ remain to be determined. To address this need, we acquired images of cytosolic Ca²⁺ transients in 1300 hepatocytes situated across several mouse liver lobules over a period of 1600 s. We analyzed this time se

SUBMITTER: Verma A 

PROVIDER: S-EPMC6180170 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature