Global analysis of sRNA target genes in Mycoplasma hyopneumoniae.

ABSTRACT:

Background

Small RNAs (sRNAs) are noncoding molecules that regulate different cellular activities in several bacteria. The role of sRNAs in gene expression regulation is poorly characterized in the etiological agent of porcine enzootic pneumonia Mycoplasma hyopneumoniae. We performed a global analysis of the sRNAs, sRNA target genes and regulatory elements previously identified in their genome and analyzed the expression of some sRNAs and their target genes by quantitative RT-PCR (qPCR) in three different culture conditions.

Results

Seven of the 145 sRNA target genes are organized as monocistronic genes (mCs) while the other 138 sRNA target genes are organized into transcriptional units (TU). The identification of transcriptional regulatory elements (promoter motif, DNA repe

SUBMITTER: Fritsch TE 

PROVIDER: S-EPMC6199787 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature