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Biopanning of allergens from wasp sting patients.

ABSTRACT: Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of the present study was to systematically study the epitopes of wasp venom. Using a random 12-peptide phage library, we performed antibody-binding epitope panning on ten serum samples from wasp sting victims at 3 h and 4 days after the sting. The panning epitopes were identified by high-throughput sequencing and matched with wasp venom proteins by BLAST. The panned antibody-binding epitopes were verified by ELISA. A total of 35 specific potential wasp venom epitopes in 4 days were identified. Amongst them, twelve peptide epitopes were matched with nine wasp venom proteins, namely, vitellogenin precursor, hexamerin 70b precursor, venom carboxylesterase-6 precursor, MRJP5, major royal jelly protein 8 precursor, venom acid phosphatase Acph-1 precursor, phospholipase A2, venom serine protease 34 precursor, and major royal jelly protein 9 precursor. The changes in serum IgM antibodies induced by wasp venom were confirmed by ELISA based on the 12 peptide epitopes. The nine wasp venom proteins are potential allergens, which should be excluded or modified in the potential biomedical applications of wasp venom.

SUBMITTER: Chai L

PROVIDER: S-EPMC6200702 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Biopanning of allergens from wasp sting patients.

Chai Lin L Yang Xianyi X Liu Mei M Liu Chunyan C Han Limei L Guo Hui H Li Changsheng C Sun Yuwen Y Li Xiaoyan X Xiao Min M Fang Zhicheng Z

Bioscience reports 20181017 5

<h4>Objective</h4>Wasp venom is a potentially important natural drug, but it can cause hypersensitivity reactions. The purpose of the present study was to systematically study the epitopes of wasp venom.<h4>Methods</h4>Using a random 12-peptide phage library, we performed antibody-binding epitope panning on ten serum samples from wasp sting victims at 3 h and 4 days after the sting. The panning epitopes were identified by high-throughput sequencing and matched with wasp venom proteins by BLAST. ...[more]

PMID: 30249752

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Project description:BackgroundIn recent years, the incidence of wasp sting has increased annually in China. Organ damage and high mortality due to mass wasp envenomation remain major challenges. Timely and appropriate medical intervention can improve survival. However, there are currently no normalized tools for early assessment of severity.MethodsThe clinical data of wasp sting patients hospitalized from 2011 to 2019 were used as a training set. Logistic regression was used to explore major risk factors for the development of a severe case of wasp sting (SC). The Wasp Sting Severity Score (WSS) was determined considering these risk factors to identify SCs and was tested in a validation dataset that was prospectively collected in 2020.ResultsThe data of 1131 wasp sting patients from 2011 to 2019 were included in the training set. Logistic regression analysis showed that tea-colored urine, number of stings, and lactate dehydrogenase and total bilirubin levels were risk factors for developing an SC. The WSS was developed considering these four risk factors, and the total possible WSS was 20 points. The WSS was tested using the validation dataset, comprising the data of 153 patients, in 2020, and we found that a WSS ≥3 points was an important indication for blood purification, with a sensitivity of 71.9%, specificity of 92.6% and an area under the curve of 0.918 (95% confidence interval 0.873-0.962). Among patients with more than 30 stings, mortality in those who underwent plasma exchange (PE) within 24 h after admission was significantly lower than that in those who did not receive PE treatment (14.3% versus 46.9%, P = 0.003). However, continuous venovenous hemofiltration (CVVH) (P = 0.317) and hemoperfusion (HP) (P = 0.869) did not significantly reduce mortality.ConclusionsPatients with WSS scores ≥3 should be considered for blood purification as early as possible in addition to routine treatment. In addition, PE is better than CVVH and HP at reducing mortality in patients suffering from severe wasp stings.

Project description:The vast majority of species of velvet ants (Hymenoptera: Aculeata: Mutillidae) are ectoparasitoids of immature stages of other aculeate Hymenoptera (bees, wasps and ants). Due to their cryptic, furtive behaviour at the host nesting sites, however, even basic information on their biology, like host use diversity, is still unknown for entire subfamilies, and the known information, scattered in over two centuries of published studies, is potentially hiding tendencies to host specialization across velvet ant lineages. In this review, based on 305 host associations spanning 132 species in 49 genera and 10 main lineages (tribes/subfamilies), we explored patterns of host use in velvet ants. Overall, 15 families and 29 subfamilies of Aculeata are listed as hosts of mutillids, with a strong predominance of Apoidea (bees and apoid wasps: 19 subfamilies and 82.3% of host records). A series of bipartite networks, multivariate analyses and calculations of different indices suggested possible patterns of specialization. Host taxonomic spectrum (number of subfamilies) of velvet ants was very variable and explained by variation in the number of host records. Instead, we found a great variation of network-based host specialization degree and host taxonomic distinctness that did not depend on the number of host records. Differences in host use patterns seemed apparent across mutillid tribes/subfamilies, among genera within several tribes/subfamilies, and to lesser extent within genera. Taxonomic host use variation seemed not dependent on phylogeny. Instead, it was likely driven by the exploitation of hosts with different ecological traits (nest type, larval diet and sociality). Thus, taxonomically more generalist lineages may use hosts that essentially share the same ecological profile. Interestingly, closely related mutillid lineages often show contrasting combinations of host ecological traits, particularly sociality and larval diet, with a more common preference for ground-nesting hosts across most lineages. This review may serve as a basis to test hypotheses for host use evolution in this fascinating family of parasitoids.

Dataset Information

Biopanning of allergens from wasp sting patients.

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Biopanning of allergens from wasp sting patients.

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