Development and characterization of EST-SSR markers for Carex angustisquama (Cyperaceae), an extremophyte in solfatara fields.

ABSTRACT:

Premise of the study

Expressed sequence tag-simple sequence repeat (EST-SSR) markers were developed for Carex angustisquama (Cyperaceae) to investigate the evolutionary history of this plant that is endemic to solfatara fields in northern Japan.

Methods and results

Using RNA-Seq data generated by the Illumina HiSeq 2000, 20 EST-SSR markers were developed. Polymorphisms were assessed in C. angustisquama and the closely related species C. doenitzii and C. podogyna. In C. angustisquama, many loci were monomorphic within populations; the average number of alleles ranged from one to five, and levels of expected heterozygosity ranged from 0.000 to 0.580, while all markers were polymorphic in a population of C. doenitzii. This indicates tha

SUBMITTER: Nagasawa K 

PROVIDER: S-EPMC6201717 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature