Separation and Bioactive Assay of 25R/S-Spirostanol Saponin Diastereomers from Yucca schidigera Roezl (Mojave) Stems.

ABSTRACT: In order to find a simple, generic, efficient separation method for 25R/S-spirostanol saponin diastereomers, the liquid chromatographic retention behaviors of C₁₂ carbonylation and C₁₂ unsubstituted 25R/S-spirostanol saponin diastereomers on different stationary phases (C₈, C₁₈, C₃₀ columns) and different mobile phases (MeOH-1% CH₃COOH and CH₃CN-1% CH₃COOH) were investigated. A C₃₀ column was firstly found to offer the highest efficiency for the separation of this kind of diastereomers than C₈ and C₁₈ columns. Meanwhile, the analysis results indicated that both CH₃CN-1% CH₃COOH and MeOH-1% CH₃COOH eluate systems were selective for C₁₂ unsubstituted 25R/S-spirostanol saponi

SUBMITTER: Qu L 

PROVIDER: S-EPMC6222657 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature