Co-transplantation of mesenchymal stem cells improves spermatogonial stem cell transplantation efficiency in mice.

ABSTRACT:

Background

Spermatogonial stem cell transplantation (SSCT) could become a fertility restoration tool for childhood cancer survivors. However, since in mice, the colonization efficiency of transplanted spermatogonial stem cells (SSCs) is only 12%, the efficiency of the procedure needs to be improved before clinical implementation is possible. Co-transplantation of mesenchymal stem cells (MSCs) might increase colonization efficiency of SSCs by restoring the SSC niche after gonadotoxic treatment.

Methods

A mouse model for long-term infertility was developed and used to transplant SSCs (SSCT, n = 10), MSCs (MSCT, n = 10), a combination of SSCs and MSCs (MS-SSCT, n = 10), or a combination of SSCs and TGFß1-treated MSCs (MSi-SSCT, n = 10).

Results

The best model for transpl

SUBMITTER: Kadam P 

PROVIDER: S-EPMC6249754 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature