Oxidative insults disrupt OPA1-mediated mitochondrial dynamics in cultured mammalian cells.

ABSTRACT:

Objective

To explore the impact of oxidative insults on mitochondrial dynamics. In mammalian cells, oxidative insults activate stress response pathways including inflammation, cytokine secretion, and apoptosis. Intriguingly, mitochondria are emerging as a sensitive network that may function as an early indicator of subsequent cellular stress responses. Mitochondria form a dynamic network, balancing fusion, mediated by optic atrophy-1 (OPA1), and fission events, mediated by dynamin-related protein-1 (DRP1), to maintain homeostasis.

Methods

Here, we examine the impact of oxidative insults on mitochondrial dynamics in 143B osteosarcoma and H9c2 cardiomyoblast cell lines via confocal microscopy, flow cytometry, and protein-based analyses.

Results

When challenged with hydr

SUBMITTER: Garcia I 

PROVIDER: S-EPMC6272060 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature