Accumulation of GD1? Ganglioside in MDA-MB-231 Breast Cancer Cells Expressing ST6GalNAc V.

ABSTRACT: ?-Series gangliosides define a particular sub-class of glycosphingolipids containing sialic acid ?2,6-linked to GalNAc residue that was isolated as a minor compound from the brain. The sialyltransferase ST6GalNAc V was cloned from mouse brain and showed ?2,6-sialyltransferase activity almost exclusively for GM1b, to form GD1? and is considered as the main enzyme involved in the biosynthesis of ?-series gangliosides. Recently, ST6GALNAC5 was identified as one of the genes over-expressed in breast cancer cell populations selected for their ability to produce brain metastasis. However, the capacity of human breast cancer cells to produce ?-series gangliosides has never been clearly demonstrated. Here, we show by stable transfection and MS-MS analysis of total glycosphingolipids that ST6GALNAC5 expressing MDA-MB-231 breast cancer cells accumulate GD1? ganglioside (IV3Neu5Ac1, III6Neu5Ac1Gg4-Cer).

SUBMITTER: Vandermeersch S 

PROVIDER: S-EPMC6272744 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature