Unknown

Dataset Information

0

Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient.


ABSTRACT: Enteroendocrine cells (EECs) control a wide range of physiological processes linked to metabolism1. We show that EEC hormones are differentially expressed between crypts (for example, Glp1) and villi (for example, secretin). As demonstrated by single-cell mRNA sequencing using murine Lgr5+ cell-derived organoids, BMP4 signals alter the hormone expression profiles of individual EECs to resemble those found in the villus. Accordingly, BMP4 induces hormone switching of EECs migrating up the crypt-villus axis in vivo. Our findings imply that EEC lineages in the small intestine exhibit a more flexible hormone repertoire than previously proposed. We also describe a protocol to generate human EECs in organoids and demonstrate a similar regulation of hormone expression by BMP signalling. These findings establish alternative strategies to target EECs with therapeutically relevant hormone production through BMP modulation.

SUBMITTER: Beumer J 

PROVIDER: S-EPMC6276989 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Enteroendocrine cells switch hormone expression along the crypt-to-villus BMP signalling gradient.

Beumer Joep J   Artegiani Benedetta B   Post Yorick Y   Reimann Frank F   Gribble Fiona F   Nguyen Thuc Nghi TN   Zeng Hongkui H   Van den Born Maaike M   Van Es Johan H JH   Clevers Hans H  

Nature cell biology 20180723 8


Enteroendocrine cells (EECs) control a wide range of physiological processes linked to metabolism<sup>1</sup>. We show that EEC hormones are differentially expressed between crypts (for example, Glp1) and villi (for example, secretin). As demonstrated by single-cell mRNA sequencing using murine Lgr5<sup>+</sup> cell-derived organoids, BMP4 signals alter the hormone expression profiles of individual EECs to resemble those found in the villus. Accordingly, BMP4 induces hormone switching of EECs mi  ...[more]

Similar Datasets

2022-05-02 | GSE194004 | GEO
| S-EPMC4890533 | biostudies-literature
| S-EPMC4997266 | biostudies-literature
| S-EPMC4393184 | biostudies-literature
2009-04-05 | GSE15421 | GEO
| S-EPMC1186529 | biostudies-other
2005-11-29 | GSE3216 | GEO
2023-11-08 | GSE244918 | GEO
| S-EPMC5526999 | biostudies-literature
| S-EPMC1130550 | biostudies-other