A combined microfluidic-transcriptomic approach to characterize the extravasation potential of cancer cells.

ABSTRACT: The reciprocal interaction between circulating tumor cells (CTCs) and tissue-specific cells is influential for the progression of metastases. In particular, the process of extravasation relies on the complex cross-talk between cancer cells and other cellular players such as the endothelium and the secondary tissue. However, most in vitro studies only focus on one heterotypic cell-cell interaction and often lack of physiological relevance. In this project, we investigated both CTC-endothelium and CTC-secondary site interactions during cancer cell extravasation. We first used a microarray analysis of extravasated MDA-MB-231 breast cancer cells to identify key markers involved in extravasation. Then, we developed a tri-culture microfluidic platform combining cancer cells, endothelium a

SUBMITTER: Bersini S 

PROVIDER: S-EPMC6281425 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature