Rapid antibiotic susceptibility testing from blood culture bottles with species agnostic real-time polymerase chain reaction.

ABSTRACT: Development and implementation of rapid antimicrobial susceptibility testing is critical for guiding patient care and improving clinical outcomes, especially in cases of sepsis. One approach to reduce the time-to-answer for antimicrobial susceptibility is monitoring the inhibition of DNA production, as differences in DNA concentrations are more quickly impacted compared to optical density changes in traditional antimicrobial susceptibility testing. Here, we use real-time PCR to rapidly determine antimicrobial susceptibility after short incubations with antibiotic. Application of this assay to a collection of 144 isolates in mock blood culture, covering medically relevant pathogens displaying high rates of resistance, provided susceptibility data in under 4 hours. This assay provided catego

SUBMITTER: Maxson T 

PROVIDER: S-EPMC6292663 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature