Unknown

Dataset Information

0

Tissue engineering of human hair follicles using a biomimetic developmental approach.


ABSTRACT: Human skin constructs (HSCs) have the potential to provide an effective therapy for patients with significant skin injuries and to enable human-relevant drug screening for skin diseases; however, the incorporation of engineered skin appendages, such as hair follicles (HFs), into HSCs remains a major challenge. Here, we demonstrate a biomimetic approach for generation of human HFs within HSCs by recapitulating the physiological 3D organization of cells in the HF microenvironment using 3D-printed molds. Overexpression of Lef-1 in dermal papilla cells (DPC) restores the intact DPC transcriptional signature and significantly enhances the efficiency of HF differentiation in HSCs. Furthermore, vascularization of hair-bearing HSCs prior to engraftment allows for efficient human hair growth in immunodeficient mice. The ability to regenerate an entire HF from cultured human cells will have a transformative impact on the medical management of different types of alopecia, as well as chronic wounds, which represent major unmet medical needs.

SUBMITTER: Abaci HE 

PROVIDER: S-EPMC6294003 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

Tissue engineering of human hair follicles using a biomimetic developmental approach.

Abaci Hasan Erbil HE   Coffman Abigail A   Doucet Yanne Y   Chen James J   Jacków Joanna J   Wang Etienne E   Guo Zongyou Z   Shin Jung U JU   Jahoda Colin A CA   Christiano Angela M AM  

Nature communications 20181213 1


Human skin constructs (HSCs) have the potential to provide an effective therapy for patients with significant skin injuries and to enable human-relevant drug screening for skin diseases; however, the incorporation of engineered skin appendages, such as hair follicles (HFs), into HSCs remains a major challenge. Here, we demonstrate a biomimetic approach for generation of human HFs within HSCs by recapitulating the physiological 3D organization of cells in the HF microenvironment using 3D-printed  ...[more]

Similar Datasets

| S-EPMC4863197 | biostudies-literature
| S-EPMC6321415 | biostudies-literature
| S-EPMC6115422 | biostudies-literature
| S-EPMC6128885 | biostudies-other
| S-EPMC7279462 | biostudies-literature
| S-EPMC1770800 | biostudies-other
| S-EPMC5749123 | biostudies-literature
| S-EPMC6109114 | biostudies-literature
| S-EPMC6743420 | biostudies-literature
| S-EPMC8478231 | biostudies-literature