Explant culture of oral mucosal epithelial cells for fabricating transplantable epithelial cell sheet.

ABSTRACT:

Introduction

Carrier-free autologous mucosal epithelial cell sheets have been clinically utilized as a cell therapy for various epithelial disorders. Fabrication of a transplantable oral mucosal epithelial cell sheet without mouse feeder layers requires a higher seeding density than that of a sheet with mouse feeder layer culture; therefore, a large amount of donor mucosal tissue is needed. However, cell grafts co-cultured with mouse feeder layers are classified by the US Food and Drug Administration (FDA) as xenogeneic products. The goal of this study was to evaluate the utility of oral mucosal epithelial cells expanded by primary explant culture for the fabrication of an adequate number of transplantable epithelial cell sheets without mouse feeder layers.

Methods

Small fra

SUBMITTER: Morino T 

PROVIDER: S-EPMC6298907 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature