A Liposomal Platform for Sensing of Extracellular Analytes Near Cells.

ABSTRACT: Cell-permeable fluorescent chemosensors (calcein, monochlorobimane, and a recently reported spiropyran-based sensor SP2) have been incorporated into yeast total lipid extract-based liposomes to suppress inherent cell permeability to allow the detection of extracellular Ca²⁺, GSH, and Zn²⁺, respectively. The repurposed sensors have enhanced aqueous solubility and the ability to quantitatively measure biologically relevant concentrations of Ca²⁺ (0.25 mM⁻1 mM), Zn²⁺ (6.25 µM⁻50 µM), and GSH (0.25 mM⁻1 mM) by fluorescence in aqueous media. In addition, the liposomal sensors are nontoxic to HEK293 cells and have the ability to detect exogenously added Zn²⁺ (1 mM), Ca²⁺ (1 mM), or GSH (1 mM) near cells without internalisation. Th

SUBMITTER: Zhang X 

PROVIDER: S-EPMC6315562 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature