Isolation and characterization of a new Methanoculleus bourgensis strain KOR-2 from the rumen of Holstein steers.

ABSTRACT:

Objective

To isolate and identify new methanogens from the rumen of Holstein steers in Korea.

Methods

Representative rumen contents were obtained from three ruminally cannulated Holstein steers (793±8 kg). Pre-reduced media were used for the growth and isolation of methanogens. Optimum growth temperature, pH, and sodium chloride (NaCl) concentration as well as substrate utilization and antibiotic tolerance were investigated to determine the physiological characteristics of the isolated strain. Furthermore, the isolate was microscopically studied for its morphology. Polymerase chain reaction of 16S rRNA and mcrA gene-based amplicons was used for identification.

Results

One strain designated as KOR-2 was isolated and found to be a non-motile irregular coccus with a diam

SUBMITTER: Battumur U 

PROVIDER: S-EPMC6325391 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature