TMT-Based Quantitative Proteomics Analysis Reveals Airborne PM_2.5-Induced Pulmonary Fibrosis.

ABSTRACT: Epidemiological and experimental studies have documented that long-term exposure to fine particulate matter (PM_2.5) increases the risk of respiratory diseases. However, the details of the underlying mechanism remain unclear. In this study, male C57BL/6 mice were exposed to ambient PM_2.5 (mean daily concentration ~64 µg/m³) for 12 weeks through a "real-world" airborne PM_2.5 exposure system. We found that PM_2.5 caused severe lung injury in mice as evidenced by histopathological examination. Then, tandem mass tag (TMT) labeling quantitative proteomic technology was performed to analyze protein expression profiling in the lungs from control and PM_2.5-exposed mice. A total of 32 proteins were differentially expressed in PM_2.5-exp

SUBMITTER: Liu S 

PROVIDER: S-EPMC6339163 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature