Implantation initiation of self-assembled embryo-like structures generated using three types of mouse blastocyst-derived stem cells.

ABSTRACT: Spatially ordered embryo-like structures self-assembled from blastocyst-derived stem cells can be generated to mimic embryogenesis in vitro. However, the assembly system and developmental potential of such structures needs to be further studied. Here, we devise a nonadherent-suspension-shaking system to generate self-assembled embryo-like structures (ETX-embryoids) using mouse embryonic, trophoblast and extra-embryonic endoderm stem cells. When cultured together, the three cell types aggregate and sort into lineage-specific compartments. Signaling among these compartments results in molecular and morphogenic events that closely mimic those observed in wild-type embryos. These ETX-embryoids exhibit lumenogenesis, asymmetric patterns of gene expression for markers of mesoderm and primordial

SUBMITTER: Zhang S 

PROVIDER: S-EPMC6353907 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature