MALAT1: A regulator of inflammatory cytokines in diabetic complications.

ABSTRACT:

Objectives and design

In this study, we examined the role of MALAT1, a highly conserved nuclear long non-coding RNA molecule, in chronic diabetic complications affecting the heart and kidneys using both in vitro and in vivo models: human endothelial cell culture and a Malat1 knockout mice model.

Results

Findings from our in vitro experiments demonstrated that MALAT1 was predominantly localized to nuclear speckles in endothelial cells and MALAT1 expression was significantly increased following incubation with high glucose in association with increased expression of inflammatory cytokines. As for our in vivo experiments, we used Malat1 knockout mice and wild-type controls with or without streptozotocin-induced diabetes over 2 months of follow-up, where all of our diabetic anim

SUBMITTER: Gordon AD 

PROVIDER: S-EPMC6354803 | biostudies-literature | 2018 Apr

REPOSITORIES: biostudies-literature