Increased nicotinamide adenine dinucleotide pool promotes colon cancer progression by suppressing reactive oxygen species level.

ABSTRACT: Nicotinamide adenine dinucleotide (NAD) exists in an oxidized form (NAD⁺ ) and a reduced form (NADH). NAD⁺ plays crucial roles in cancer metabolism, including in cellular signaling, energy production and redox regulation. However, it remains unclear whether NAD(H) pool size (NAD⁺ and NADH) could be used as biomarker for colon cancer progression. Here, we showed that the NAD(H) pool size and NAD⁺ /NADH ratio both increased during colorectal cancer (CRC) progression due to activation of the NAD⁺ salvage pathway mediated by nicotinamide phosphoribosyltransferase (NAMPT). The NAMPT expression was upregulated in adenoma and adenocarcinoma tissues from CRC patients. The NADH fluorescence intensity measured by two-photon excitation fluorescence (TPEF) microscopy was consistently increased in CRC cell lines, azoxymethane/dextran sodium sulfate (AOM/DSS)-induced CRC tissues and tumor tissues from CRC patients. The increases in the NAD(H) pool inhibited the accumulation of excessive reactive oxygen species (ROS) levels and FK866, a specific inhibitor of NAMPT, treatment decreased the CRC nodule size by increasing ROS levels in AOM/DSS mice. Collectively, our results suggest that NAMPT-mediated upregulation of the NAD(H) pool protects cancer cells against detrimental oxidative stress and that detecting NADH fluorescence by TPEF microscopy could be a potential method for monitoring CRC progression.

SUBMITTER: Hong SM 

PROVIDER: S-EPMC6361564 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature