A porcine model to study the effect of brain death on kidney genomic responses.

ABSTRACT:

Introduction

A majority of transplanted organs come from donors after brain death (BD). Renal grafts from these donors have higher delayed graft function and lower long-term survival rates compared to living donors. We designed a novel porcine BD model to better delineate the incompletely understood inflammatory response to BD, hypothesizing that adhesion molecule pathways would be upregulated in BD.

Methods

Animals were anesthetized and instrumented with monitors and a balloon catheter, then randomized to control and BD groups. BD was induced by inflating the balloon catheter and animals were maintained for 6 hours. RNA was extracted from kidneys, and gene expression pattern was determined.

Results

In total, 902 gene pairs were differently expressed between groups. E

SUBMITTER: Sally MB 

PROVIDER: S-EPMC6374499 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature