Anoctamin-4 is a bona fide Ca²⁺-dependent non-selective cation channel.

ABSTRACT: Changes in cell function occur by specific patterns of intracellular Ca²⁺, activating Ca²⁺-sensitive proteins. The anoctamin (TMEM16) protein family has Ca²⁺-dependent ion channel activity, which provides transmembrane ion transport, and/or Ca²⁺-dependent phosphatidyl-scramblase activity. Using amino acid sequence analysis combined with measurements of ion channel function, we clarified the so far unknown Ano4 function as Ca²⁺-dependent, non-selective monovalent cation channel; heterologous Ano4 expression in HEK293 cells elicits Ca²⁺ activated conductance with weak selectivity of K⁺ > Na⁺ > Li⁺. Endogenously expressed Ca²⁺-dependent cation channels in the retinal pigment epitheliu

SUBMITTER: Reichhart N 

PROVIDER: S-EPMC6381168 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature