Development of 15 microsatellite loci in the endangered Streptanthus glandulosus subsp. niger (Brassicaceae).

ABSTRACT:

Premise of the study

The endangered Streptanthus glandulosus subsp. niger (Brassicaceae) is endemic to a single peninsula in California and threatened by fragmentation. We developed microsatellite markers to investigate genetic diversity in the two extant populations and the degree to which they have diverged from one another.

Methods and results

We used Illumina HiSeq high-throughput sequencing to develop 15 microsatellite markers, 14 of which were polymorphic. These di- and trinucleotide repeats yielded one to 11 alleles per locus in 61 plants across the two populations. Levels of observed and expected heterozygosities ranged from 0.108 to 0.946 and 0.257 to 0.839, respectively. We demonstrated cross-amplification in a second rare subspecies, S. glandulosu

SUBMITTER: Swope SM 

PROVIDER: S-EPMC6384297 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature