Bifunctional cleavable probes for in situ multiplexed glycan detection and imaging using mass spectrometry.

ABSTRACT: In situ analysis of glycans is of great significance since they mediate a range of biological activities. Aberrant changes of glycosylation are closely related to cancer onset and progression. In this work, bifunctional laser cleavable mass probes (LCMPs) were developed for in situ glycan detection from both cells and tissues using laser desorption ionization mass spectrometry (LDI-MS). Specific recognition of glycans was achieved by lectins, and inherent signal amplification was achieved by the conversion of the detection of glycans to that of mass tags which overcame the low ionization efficiency and complicated mass spectra of glycans. Multiplexed glycan profiling was easy to implement due to the simple and generic synthetic route to LCMPs and serial alternative mass tags,

SUBMITTER: Ma W 

PROVIDER: S-EPMC6385553 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature