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Anti-Inflammatory Activity of ?-thymosin Peptide Derived from Pacific Oyster (Crassostrea gigas) on NO and PGE? Production by Down-Regulating NF-?B in LPS-Induced RAW264.7 Macrophage Cells.

ABSTRACT: ?-thymosin is known for having 43 amino acids, being water-soluble, having a light molecular weight and ubiquitous polypeptide. The biological activities of ?-thymosin are diverse and include the promotion of wound healing, reduction of inflammation, differentiation of T cells and inhibition of apoptosis. Our previous studies showed that oyster ?-thymosin originated from the mantle of the Pacific oyster, Crassostrea gigas and had antimicrobial activity. In this study, we investigated the anti-inflammatory effects of oyster ?-thymosin in lipopolysaccharide (LPS)-induced RAW264.7 macrophage cells using human ?-thymosin as a control. Oyster ?-thymosin inhibited the nitric oxide (NO) production as much as human ?-thymosin in LPS-induced RAW264.7 cells. It also showed that oyster ?-thymosin suppressed the expression of prostaglandin E? (PGE?), inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Moreover, oyster ?-thymosin reduced inflammatory cytokines such as tumor necrosis factor-? (TNF-?), interleukin-1? (IL-1?) and interleukin-6 (IL-6). Oyster ?-thymosin also suppressed the nuclear translocation of phosphorylated nuclear factor-?B (NF-?B) and degradation of inhibitory ?B (I?B) in LPS-induced RAW264.7 cells. These results suggest that oyster ?-thymosin, which is derived from the mantle of the Pacific oyster, has as much anti-inflammatory effects as human ?-thymosin. Additionally, oyster ?-thymosin suppressed NO production, PGE? production and inflammatory cytokines expression via NF-?B in LPS-induced RAW264.7 cells.

SUBMITTER: Hwang D

PROVIDER: S-EPMC6409780 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature

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Publications

Anti-Inflammatory Activity of β-thymosin Peptide Derived from Pacific Oyster (<i>Crassostrea gigas</i>) on NO and PGE₂ Production by Down-Regulating NF-κB in LPS-Induced RAW264.7 Macrophage Cells.

Hwang Dukhyun D Kang Min-Jae MJ Jo Mi Jeong MJ Seo Yong Bae YB Park Nam Gyu NG Kim Gun-Do GD

Marine drugs 20190221 2

β-thymosin is known for having 43 amino acids, being water-soluble, having a light molecular weight and ubiquitous polypeptide. The biological activities of β-thymosin are diverse and include the promotion of wound healing, reduction of inflammation, differentiation of T cells and inhibition of apoptosis. Our previous studies showed that oyster β-thymosin originated from the mantle of the Pacific oyster, <i>Crassostrea gigas</i> and had antimicrobial activity. In this study, we investigated the ...[more]

PMID: 30795639

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Project description:BackgroundLow salinity is one of the main factors limiting the distribution and survival of marine species. As a euryhaline species, the Pacific oyster Crassostrea gigas is considered to be tolerant to relative low salinity. The genes that regulate C. gigas responses to osmotic stress were monitored using the next-generation sequencing of whole transcriptome with samples taken from gills. By RNAseq technology, transcript catalogs of up- and down-regulated genes were generated from the oysters exposed to low and optimal salinity seawater.Methodology/principal findingsThrough Illumina sequencing, we reported 1665 up-regulated transcripts and 1815 down-regulated transcripts. A total of 45771 protein-coding contigs were identified from two groups based on sequence similarities with known proteins. As determined by GO annotation and KEGG pathway mapping, functional annotation of the genes recovered diverse biological functions and processes. The genes that changed expression significantly were highly represented in cellular process and regulation of biological process, intracellular and cell, binding and protein binding according to GO annotation. The results highlighted genes related to osmoregulation, signaling and interactions of osmotic stress response, anti-apoptotic reactions as well as immune response, cell adhesion and communication, cytoskeleton and cell cycle.Conclusions/significanceThrough more than 1.5 million sequence reads and the expression data of the two libraries, the study provided some useful insights into signal transduction pathways in oysters and offered a number of candidate genes as potential markers of tolerance to hypoosmotic stress for oysters. In addition, the characterization of C. gigas transcriptome will not only provide a better understanding of the molecular mechanisms about the response to osmotic stress of the oysters, but also facilitate research into biological processes to find underlying physiological adaptations to hypoosmotic shock for marine invertebrates.

Dataset Information

Anti-Inflammatory Activity of ?-thymosin Peptide Derived from Pacific Oyster (Crassostrea gigas) on NO and PGE? Production by Down-Regulating NF-?B in LPS-Induced RAW264.7 Macrophage Cells.

Publications

Anti-Inflammatory Activity of β-thymosin Peptide Derived from Pacific Oyster (<i>Crassostrea gigas</i>) on NO and PGE₂ Production by Down-Regulating NF-κB in LPS-Induced RAW264.7 Macrophage Cells.

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