Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV).

ABSTRACT:

Objective

The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome analysis. In this regard, seven potential reference genes were tested by RT-qPCR and ranked according to their stability using BestKeeper, NormFinder and GeNorm algorithms, and RefFinder WEB-based software in an experiment performed with samples from two sugarcane cultivars contrasting for SCMV resistance, when mechanically inoculated with a severe SCMV strain and using mock inoculated plant controls.

Results

The genes Uridylate kinase (UK) and Ubiquitin-conjugating enzyme 18 (UBC18) were the most stable according to GeNorm algor

SUBMITTER: da Silva MF 

PROVIDER: S-EPMC6423880 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature