Dataset Information

Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma.

ABSTRACT:

Objective

To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date.

Design

Laboratory-based investigation.

Setting

Academic center.

Patient(s)

Women undergoing hysterectomy for benign indications.

Intervention(s)

Next-generation sequencing and screening of an sncRNA database with confirmatory analysis by quantitative reverse-transcription polymerase chain reaction (qRT-PCR).

Main outcome measure(s)

Expression profile of sncRNAs in leiomyoma and matched myometrium.

Result(s)

Screening our previously determined RNA sequencing data with the sncRNA database resulted in identification of 15 small nuclear (sn) RNAs, 284 small nucleolar (sno) RNAs, 98 Piwi-interacting (pi) RNAs, 152 transfer (t) RNAs, and 45 ribosomal (r) RNAs, of which 15 snoRNAs, 24 piRNAs, 7 tRNAs, and 6 rRNAs were differentially expressed at a 1.5-fold change cutoff in leiomyoma compared with myometrium. We selected 5 snoRNAs, 4 piRNAs, 1 tRNA, and 1 rRNA that were differentially expressed and confirmed their expression in paired tissues (n = 20) from both phases of the menstrual cycle with the use of qRT-PCR. The results indicated up-regulation of the snoRNAs (SNORD30, SNORD27, SNORA16A, SNORD46, and SNORD56) and down-regulation of the piRNAs (piR-1311, piR-16677, piR-20365, piR-4153), tRNA (TRG-GCC5-1), and rRNA (RNA5SP202) expression in leiomyoma compared with myometrium (P<.05). The pattern of expression of these sncRNAs was similar to RNA sequencing analysis, with no menstrual cycle-dependent differences detected except for SNORD30. Because Argonaute 2 (AGO2) is required for sncRNA-mediated gene silencing, we determined its expression and found greater abundance in leiomyoma.

Conclusion(s)

Our results provide the first evidence for the differential expression of additional classes of sncRNAs and AGO2 in leiomyoma, implicating their roles as a gene regulatory mechanism.

SUBMITTER: Chuang TD

PROVIDER: S-EPMC6445395 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma.

Chuang Tsai-Der TD Xie Yeming Y Yan Wei W Khorram Omid O

Fertility and sterility 20180501 5

<h4>Objective</h4>To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date.<h4>Design</h4>Laboratory-based investigation.<h4>Setting</h4>Academic center.<h4>Patient(s)</h4>Women undergoing hysterectomy for benign indications.<h4>Intervention(s)</h4>Next-generation sequencing and screening of an sncRNA database with confirmatory analysis by quantitative reverse-transcription polymerase chain reaction (qRT-PCR).<h4>Main outcome mea ...[more]

PMID: 29778390

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Dataset Information

Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma.

Objective

Design

Setting

Patient(s)

Intervention(s)

Main outcome measure(s)

Result(s)

Conclusion(s)

Publications

Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma.

Similar Datasets

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