Next-generation sequencing reveals differentially expressed small noncoding RNAs in uterine leiomyoma.

ABSTRACT:

Objective

To determine the expression profile of small noncoding RNAs (sncRNAs) in leiomyoma, which has not been investigated to date.

Design

Laboratory-based investigation.

Setting

Academic center.

Patient(s)

Women undergoing hysterectomy for benign indications.

Intervention(s)

Next-generation sequencing and screening of an sncRNA database with confirmatory analysis by quantitative reverse-transcription polymerase chain reaction (qRT-PCR).

Main outcome measure(s)

Expression profile of sncRNAs in leiomyoma and matched myometrium.

Result(s)

Screening our previously determined RNA sequencing data with the sncRNA database resulted in identification of 15 small nuclear (sn) RNAs, 284 small nucleolar (sno) RNAs, 98 Piwi-interacting (pi) RNAs, 15

SUBMITTER: Chuang TD 

PROVIDER: S-EPMC6445395 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature