The use of clade-specific PCR assays to identify novel nitrilase genes from environmental isolates.

ABSTRACT: Nitrilase enzymes (EC 3.5.5.1) are responsible for the direct hydration of nitriles to their corresponding carboxylic acids and ammonia. The utilization of nitrilase enzymes in biocatalysis toward bio-pharmaceuticals and industrial applications facilitates the move towards green chemistry. The body of research presented describes a novel clade-specific touchdown PCR protocol for the detection of novel nitrilase genes. The presented study identified partial sequences of 15 novel nitrilase genes across 7 genera, with partial DNA sequence homology (%) displayed across an additional 16 genera. This research will prove valuable in the screening of microorganisms for the identification of novel clade-specific nitrilase genes, with predicted enantioselective profiles as determined by their clade

SUBMITTER: Dooley-Cullinane TM 

PROVIDER: S-EPMC6460282 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature