Homogeneity of Mycoplasma agalctiae vaccine strains in an agalaxy- high-burden environment.

ABSTRACT:

Background and objectives

Interrogation of the genomic relations between Iranian Mycoplasma agalactiae vaccine strains of Taliqan, Lorestan and Shiraz.

Materials and methods

Two MLVA (covering VNTR loci of 5, 9, 17 and 19) and MLST (comprising dnaA, gltX, gyrB, C, tufA genes) genotyping systems plus nucleotide structure analysis of P80 gene, was conducted.

Results

The shared MLVA pattern represented by the three strains differed to that of the Mag PG2 laboratory strain, only at locus VNTR19 where the PG2 genome hold a 3 bp longer stretch. In MLST analysis, at dnaA, gltX, gyrB, metS and tufA loci, the three strains displayed alleles 1, 21, 2, 2 and 1, respectively. At, gltX locus a new allele (21) was detected where a ne

SUBMITTER: Kabiri K 

PROVIDER: S-EPMC6462265 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature