MicroRNA profiling of mouse liver in response to DENV-1 infection by deep sequencing.

ABSTRACT:

Background

Dengue caused by dengue virus (DENV) serotypes -1 to -4 is the most important mosquito-borne viral disease in the tropical and sub-tropical countries worldwide. Yet many of the pathophysiological mechanisms of host responses during DENV infection remain largely unknown and incompletely understood.

Methods

Using a mouse model, the miRNA expressions in liver during DENV-1 infection was investigated using high throughput miRNA sequencing. The differential expressions of miRNAs were then validated by qPCR, followed by target genes prediction. The identified miRNA targets were subjected to gene ontology (GO) annotation and pathway enrichment analysis to elucidate the potential biological pathways and molecular mechanisms associated with DENV-1 infection.

Results

SUBMITTER: Pong LY 

PROVIDER: S-EPMC6482938 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature