Remote ischemic preconditioning attenuates intestinal mucosal damage: insight from a rat model of ischemia-reperfusion injury.

ABSTRACT:

Background

Remote ischemic preconditioning (RIPC) is a phenomenon, whereby repeated, non-lethal episodes of ischemia to an organ or limb exert protection against ischemia-reperfusion (I/R) injury in distant organs. Despite intensive research, there is still an apparent lack of knowledge concerning the RIPC-mediated mechanisms, especially in the intestine. Aim of this study was to evaluate possible protective effects RIPC on intestinal I/R injury.

Methods

Thirty rats were randomly assigned to four groups: I/R; I/R?+?RIPC; Sham; Sham?+?RIPC. Animals were anesthetized and the superior mesenteric artery was clamped for 30 min, followed by 60 min of reperfusion. RIPC-treated rats received 3?×?5 min of bilateral hindlimb I/R prior to surgery, sham groups obtained laparotomy without clamping. After I/R injury serum/tissue was analyzed for: Mucosal damage, Caspase-3/7 activity, expression of cell stress proteins, hydrogen peroxide (H₂O₂) and malondialdehyde (MDA) production, Hypoxia-inducible factor-1? (HIF-1?) protein expression and matrix metalloproteinase (MMP) activity.

Results

Intestinal I/R resulted in increased mucosal injury (P?2O₂ and MDA remained unchanged after RIPC, while the reduced intestinal injury was associated with increased HIF-1? levels. Measurements of MMP activities in serum and intestinal tissue revealed an attenuated gelatinase activity at 130 kDa within the serum samples (P?ConclusionsRIPC ameliorates intestinal I/R injury in rats. The underlying mechanisms may involve HIF-1? protein expression and a decreased serum activity of a 130 kDa factor with gelatinase activity.

SUBMITTER: Hummitzsch L 

PROVIDER: S-EPMC6489261 | biostudies-literature | 2019 Apr

REPOSITORIES: biostudies-literature