Neural Progenitor Cells Rptor Ablation Impairs Development but Benefits to Seizure-Induced Behavioral Abnormalities.

ABSTRACT:

Aims

Previous study suggests that mTOR signaling pathway may play an important role in epileptogenesis. The present work was designed to explore the contribution of raptor protein to the development of epilepsy and comorbidities.

Methods

Mice with conditional knockout of raptor protein were generated by cross-bred Rptor^flox/flox mice with nestin-CRE mice. The expression of raptor protein was analyzed by Western blotting in brain tissue samples. Neuronal death and mossy fiber sprouting were detected by FJB staining and Timm staining, respectively. Spontaneous seizures were recorded by EEG-video system. Morris water maze, open field test, and excitability test were used to study the behaviors of Rptor CKO mice.

Results

As the consequence of deleting Rptor, do

SUBMITTER: Chen LL 

PROVIDER: S-EPMC6492873 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature