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Data of antibacterial activity of plant leaves crude extract on bacterial isolates of wound infections.

ABSTRACT: Wound infections are caused by various pathogenic microbes such as S. aureus, Non-coagulase Streptococcus, Enterococci, E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter, Streptococci, Candida and Acinetobacter. 10-33% of septic wounds infections were seen in India. Multi-drug resistant bacterial infections are increased by day by day and these organisms showed resistant to most available antibiotics. Drug resistance is a common and natural mechanism in microorganisms, because of unbearable use of antibiotics. In this data provides the use of natural plant leaf extracts as alternatives to the multi-drug resistant bacteria. The present article contains the data on the antimicrobial activity of methanol extracts of plant leaves comprising of 11 natural plant species which are widely used as folk medicine. The leaf extracts were used against multi drug resistant bacterial isolates of septic wound infections which were evaluated by the Kirby-beur disk diffusion method. This data showed that among 11 plant methanol leaf extracts; Punica granatum and Syzigium cumini have the potential antibacterial activity against the predominant bacterial isolates of septic wounds that are MDR-P. aeruginosa, S. aureus, K. pneumoniae and E. coli.

SUBMITTER: Pallavali RR

PROVIDER: S-EPMC6495087 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature

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Data of antibacterial activity of plant leaves crude extract on bacterial isolates of wound infections.

Pallavali Roja Rani RR Pallavali Roja Rani RR Avula Srinu S Degati Vijaya Lakshmi VL Penubala Mohan M Damu A G AG Durbaka Vijaya Raghava Prasad VRP

Data in brief 20190422

Wound infections are caused by various pathogenic microbes such as S. aureus, Non-coagulase Streptococcus, Enterococci, E. coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Enterobacter, Streptococci, Candida and Acinetobacter. 10-33% of septic wounds infections were seen in India. Multi-drug resistant bacterial infections are increased by day by day and these organisms showed resistant to most available antibiotics. Drug resistance is a common and natural mechanism in mi ...[more]

PMID: 31065580

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Project description:Multi-drug resistance has become a major problem for the treatment of pathogenic bacterial infections. The use of bacteriophages is an attractive approach to overcome the problem of drug resistance in several pathogens that cause fatal diseases. Our study aimed to isolate multi drug resistant bacteria from patients with septic wounds and then isolate and apply bacteriophages in vitro as alternative therapeutic agents. Pus samples were aseptically collected from Rajiv Gandhi Institute of Medical Science (RIMS), Kadapa, A.P., and samples were analyzed by gram staining, evaluating morphological characteristics, and biochemical methods. MDR-bacterial strains were collected using the Kirby-Bauer disk diffusion method against a variety of antibiotics. Bacteriophages were collected and tested in vitro for lytic activity against MDR-bacterial isolates. Analysis of the pus swab samples revealed that the most of the isolates detected had Pseudomonas aeruginosa as the predominant bacterium, followed by Staphylococcus aureus, Klebsiella pneumoniae and Escherichia coli. Our results suggested that gram-negative bacteria were more predominant than gram-positive bacteria in septic wounds; most of these isolates were resistant to ampicillin, amoxicillin, penicillin, vancomycin and tetracycline. All the gram-positive isolates (100%) were multi-drug resistant, whereas 86% of the gram-negative isolates had a drug resistant nature. Further bacteriophages isolated from sewage demonstrated perfect lytic activity against the multi-drug resistant bacteria causing septic wounds. In vitro analysis of the isolated bacteriophages demonstrated perfect lysis against the corresponding MDR-bacteria, and these isolated phages may be promising as a first choice for prophylaxis against wound sepsis, Moreover, phage therapy does not enhance multi-drug resistance in bacteria and could work simultaneously on a wide variety of MDR-bacteria when used in a bacteriophage cocktail. Hence, our results suggest that these bacteriophages could be potential therapeutic options for treating septic wounds caused by P. aeruginosa, S. aureus, K. pneumoniae and E. coli.

Project description:Wound healing is a complex process that involves several biological events, and a delay in this process may cause economic and social problems for the patient. The search continues for new alternative treatments to aid healing, including the use of herbal medicines. Members of the genus Caesalpinia are used in traditional medicine to treat wounds. The related species Poincianella pluviosa (DC.) L.P. Queiroz increases the cell viability of keratinocytes and fibroblasts and stimulates the proliferation of keratinocytes in vitro. The crude extract (CE) from bark of P. pluviosa was evaluated in the wound-healing process in vivo, to validate the traditional use and the in vitro activity. Standardized CE was incorporated into a gel and applied on cutaneous wounds (TCEG) and compared with the formulation without CE (Control) for 4, 7, 10, or 14 days of treatment. The effects of the CE on wound re-epithelialization; cell proliferation; permeation, using photoacoustic spectroscopy (PAS); and proteins, including vascular endothelial growth factor (VEGF), superoxide dismutase 2 (SOD-2) and cyclooxygenase 2 (COX-2) were evaluated. The TCEG stimulated the migration of keratinocytes at day 4 and proliferation on the following days, with a high concentration of cells in metaphase at 7 days. Type I collagen formed more rapidly in the TCEG. PAS showed that the CE had permeated through the skin. TCEG stimulated VEGF at day 4 and SOD-2 and COX-2 at day 7. The results suggest that the CE promoted the regulation of proteins and helped to accelerate the processes involved in healing, promoting early angiogenesis. This led to an increase in the re-epithelialized surface, with significant mitotic activity. Maturation of collagen fibers was also enhanced, which may affect the resistance of the extracellular matrix. PAS indicated a correlation between the rate of diffusion and biological events during the healing process. The CE from P. pluviosa appears promising as an aid in healing.

Dataset Information

Data of antibacterial activity of plant leaves crude extract on bacterial isolates of wound infections.

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Data of antibacterial activity of plant leaves crude extract on bacterial isolates of wound infections.

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